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well primary antibodies  (R&D Systems)


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    R&D Systems well primary antibodies
    Well Primary Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/well+primary+antibodies/pm39720957-362-20-27?v=R%26D+Systems
    Average 93 stars, based on 26 article reviews
    well primary antibodies - by Bioz Stars, 2026-08
    93/100 stars

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    well primary antibodies  (R&D Systems)
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    Well Primary Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    primary antibodies for slc7a11, gpx4, and gapdh, as well as the goat anti-rabbit igg secondary antibody  (ABclonal Biotechnology)
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    primary antibodies for nhe3, tsc, and bsc-1, as well as α, β, and γ-enac  (Alpha Diagnostics)
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    Representative semiquantitative immunoblotting of kidney: ( a ) BSC-1, ( b ) Type 3 Na + /H + exchanger (NHE3), ( c ) TSC, ( d ) Na + -K + -ATPase, ( e ) <t>α-ENaC,</t> ( f ) β-ENaC, and ( g ) <t>γ-ENaC.</t> DU, densitometric unit; P, positive control; BSC-1, bumetanide-sensitive cotransporter type 1; TSC, thiazide-sensitive Na-Cl cotransporter; ENaC, epithelial sodium channel. * p < 0.05, compared to the control group. @ p < 0.05, compared to the SD group. # p < 0.05, compared to the EG group.
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    buffer of the primary antibody and secondary antibody as well as blocking buffer  (Beyotime)
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    Representative semiquantitative immunoblotting of kidney: ( a ) BSC-1, ( b ) Type 3 Na + /H + exchanger (NHE3), ( c ) TSC, ( d ) Na + -K + -ATPase, ( e ) <t>α-ENaC,</t> ( f ) β-ENaC, and ( g ) <t>γ-ENaC.</t> DU, densitometric unit; P, positive control; BSC-1, bumetanide-sensitive cotransporter type 1; TSC, thiazide-sensitive Na-Cl cotransporter; ENaC, epithelial sodium channel. * p < 0.05, compared to the control group. @ p < 0.05, compared to the SD group. # p < 0.05, compared to the EG group.
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    96-well plates with nitrocellulose membranes precoated with a primary anti-ifn-gamma antibody  (Millipore)
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    Millipore 96-well plates with nitrocellulose membranes precoated with a primary anti-ifn-gamma antibody
    Response of cytotoxic T lymphocytes stimulated by neoantigen peptide-pulsed mature dendritic cells (mDCs). ( a ) <t>IFN-γ</t> ELISpot response of peripheral blood lymphocytes stimulated by neoantigen peptide-pulsed mDCs. The final concentration of peptide was 50 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( b ) Dose-dependent increase in the IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by peptide 3- or 9-pulsed mDCs. The final concentrations of peptides were 5, 50, and 100 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( c ) Cytotoxicity assay of CTLs induced by 4 weeks of coculture with neoantigen-pulsed mDCs. Numbers of calcein-positive living tumour cells were significantly decreased by co-culture with CTLs stimulated with peptide 9-pulsed DCs. The mean % of cytotoxicity ± SEM is shown (n = 3–5). ( d ) IFNγ-release responses by peptide 9-pulsed mDC stimulated with CTLs against autologous tumour cells. Ly: lymphocyte; mDC, mature dendritic cell; TC, tumour cell; CTL/P9, CTLs induced by peptide 9-pulsed DCs; CTL/WT, CTLs induced by wildtype peptide-pulsed DCs. The mean number of IFN-γ ELISpot ± SEM. is shown (n = 2–3).
    96 Well Plates With Nitrocellulose Membranes Precoated With A Primary Anti Ifn Gamma Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated primary antibody per well  (Proteintech)
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    Response of cytotoxic T lymphocytes stimulated by neoantigen peptide-pulsed mature dendritic cells (mDCs). ( a ) <t>IFN-γ</t> ELISpot response of peripheral blood lymphocytes stimulated by neoantigen peptide-pulsed mDCs. The final concentration of peptide was 50 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( b ) Dose-dependent increase in the IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by peptide 3- or 9-pulsed mDCs. The final concentrations of peptides were 5, 50, and 100 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( c ) Cytotoxicity assay of CTLs induced by 4 weeks of coculture with neoantigen-pulsed mDCs. Numbers of calcein-positive living tumour cells were significantly decreased by co-culture with CTLs stimulated with peptide 9-pulsed DCs. The mean % of cytotoxicity ± SEM is shown (n = 3–5). ( d ) IFNγ-release responses by peptide 9-pulsed mDC stimulated with CTLs against autologous tumour cells. Ly: lymphocyte; mDC, mature dendritic cell; TC, tumour cell; CTL/P9, CTLs induced by peptide 9-pulsed DCs; CTL/WT, CTLs induced by wildtype peptide-pulsed DCs. The mean number of IFN-γ ELISpot ± SEM. is shown (n = 2–3).
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    Response of cytotoxic T lymphocytes stimulated by neoantigen peptide-pulsed mature dendritic cells (mDCs). ( a ) <t>IFN-γ</t> ELISpot response of peripheral blood lymphocytes stimulated by neoantigen peptide-pulsed mDCs. The final concentration of peptide was 50 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( b ) Dose-dependent increase in the IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by peptide 3- or 9-pulsed mDCs. The final concentrations of peptides were 5, 50, and 100 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( c ) Cytotoxicity assay of CTLs induced by 4 weeks of coculture with neoantigen-pulsed mDCs. Numbers of calcein-positive living tumour cells were significantly decreased by co-culture with CTLs stimulated with peptide 9-pulsed DCs. The mean % of cytotoxicity ± SEM is shown (n = 3–5). ( d ) IFNγ-release responses by peptide 9-pulsed mDC stimulated with CTLs against autologous tumour cells. Ly: lymphocyte; mDC, mature dendritic cell; TC, tumour cell; CTL/P9, CTLs induced by peptide 9-pulsed DCs; CTL/WT, CTLs induced by wildtype peptide-pulsed DCs. The mean number of IFN-γ ELISpot ± SEM. is shown (n = 2–3).
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    primary antibodies against the pparg as well as nfkb p65 and p50  (Cayman Chemical)
    90
    Cayman Chemical primary antibodies against the pparg as well as nfkb p65 and p50
    Response of cytotoxic T lymphocytes stimulated by neoantigen peptide-pulsed mature dendritic cells (mDCs). ( a ) <t>IFN-γ</t> ELISpot response of peripheral blood lymphocytes stimulated by neoantigen peptide-pulsed mDCs. The final concentration of peptide was 50 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( b ) Dose-dependent increase in the IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by peptide 3- or 9-pulsed mDCs. The final concentrations of peptides were 5, 50, and 100 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( c ) Cytotoxicity assay of CTLs induced by 4 weeks of coculture with neoantigen-pulsed mDCs. Numbers of calcein-positive living tumour cells were significantly decreased by co-culture with CTLs stimulated with peptide 9-pulsed DCs. The mean % of cytotoxicity ± SEM is shown (n = 3–5). ( d ) IFNγ-release responses by peptide 9-pulsed mDC stimulated with CTLs against autologous tumour cells. Ly: lymphocyte; mDC, mature dendritic cell; TC, tumour cell; CTL/P9, CTLs induced by peptide 9-pulsed DCs; CTL/WT, CTLs induced by wildtype peptide-pulsed DCs. The mean number of IFN-γ ELISpot ± SEM. is shown (n = 2–3).
    Primary Antibodies Against The Pparg As Well As Nfkb P65 And P50, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/well+primary+antibodies/pm27329536-101-31-32?v=Cayman+Chemical
    Average 90 stars, based on 1 article reviews
    primary antibodies against the pparg as well as nfkb p65 and p50 - by Bioz Stars, 2026-08
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    Image Search Results


    Representative semiquantitative immunoblotting of kidney: ( a ) BSC-1, ( b ) Type 3 Na + /H + exchanger (NHE3), ( c ) TSC, ( d ) Na + -K + -ATPase, ( e ) α-ENaC, ( f ) β-ENaC, and ( g ) γ-ENaC. DU, densitometric unit; P, positive control; BSC-1, bumetanide-sensitive cotransporter type 1; TSC, thiazide-sensitive Na-Cl cotransporter; ENaC, epithelial sodium channel. * p < 0.05, compared to the control group. @ p < 0.05, compared to the SD group. # p < 0.05, compared to the EG group.

    Journal: International Journal of Molecular Sciences

    Article Title: Long-Term Sodium Deficiency Reduces Sodium Excretion but Impairs Renal Function and Increases Stone Formation in Hyperoxaluric Calcium Oxalate Rats

    doi: 10.3390/ijms25073942

    Figure Lengend Snippet: Representative semiquantitative immunoblotting of kidney: ( a ) BSC-1, ( b ) Type 3 Na + /H + exchanger (NHE3), ( c ) TSC, ( d ) Na + -K + -ATPase, ( e ) α-ENaC, ( f ) β-ENaC, and ( g ) γ-ENaC. DU, densitometric unit; P, positive control; BSC-1, bumetanide-sensitive cotransporter type 1; TSC, thiazide-sensitive Na-Cl cotransporter; ENaC, epithelial sodium channel. * p < 0.05, compared to the control group. @ p < 0.05, compared to the SD group. # p < 0.05, compared to the EG group.

    Article Snippet: Primary antibodies for NHE3, TSC, and BSC-1, as well as α, β, and γ-ENaC, were purchased from Alpha Diagnostic International (San Antonio, TX, USA); beta-actin was purchased from Sigma Chemical Co. (St. Louis, MO, USA), and AQP was purchased from Alomone Labs Ltd. (Jerusalem, Israel).

    Techniques: Western Blot, Positive Control, Control

    Response of cytotoxic T lymphocytes stimulated by neoantigen peptide-pulsed mature dendritic cells (mDCs). ( a ) IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by neoantigen peptide-pulsed mDCs. The final concentration of peptide was 50 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( b ) Dose-dependent increase in the IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by peptide 3- or 9-pulsed mDCs. The final concentrations of peptides were 5, 50, and 100 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( c ) Cytotoxicity assay of CTLs induced by 4 weeks of coculture with neoantigen-pulsed mDCs. Numbers of calcein-positive living tumour cells were significantly decreased by co-culture with CTLs stimulated with peptide 9-pulsed DCs. The mean % of cytotoxicity ± SEM is shown (n = 3–5). ( d ) IFNγ-release responses by peptide 9-pulsed mDC stimulated with CTLs against autologous tumour cells. Ly: lymphocyte; mDC, mature dendritic cell; TC, tumour cell; CTL/P9, CTLs induced by peptide 9-pulsed DCs; CTL/WT, CTLs induced by wildtype peptide-pulsed DCs. The mean number of IFN-γ ELISpot ± SEM. is shown (n = 2–3).

    Journal: Scientific Reports

    Article Title: Neoantigens elicit T cell responses in breast cancer

    doi: 10.1038/s41598-021-91358-1

    Figure Lengend Snippet: Response of cytotoxic T lymphocytes stimulated by neoantigen peptide-pulsed mature dendritic cells (mDCs). ( a ) IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by neoantigen peptide-pulsed mDCs. The final concentration of peptide was 50 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( b ) Dose-dependent increase in the IFN-γ ELISpot response of peripheral blood lymphocytes stimulated by peptide 3- or 9-pulsed mDCs. The final concentrations of peptides were 5, 50, and 100 µg/ml. The mean number of IFN-γ ELISpots ± SEM is shown (n = 2). ( c ) Cytotoxicity assay of CTLs induced by 4 weeks of coculture with neoantigen-pulsed mDCs. Numbers of calcein-positive living tumour cells were significantly decreased by co-culture with CTLs stimulated with peptide 9-pulsed DCs. The mean % of cytotoxicity ± SEM is shown (n = 3–5). ( d ) IFNγ-release responses by peptide 9-pulsed mDC stimulated with CTLs against autologous tumour cells. Ly: lymphocyte; mDC, mature dendritic cell; TC, tumour cell; CTL/P9, CTLs induced by peptide 9-pulsed DCs; CTL/WT, CTLs induced by wildtype peptide-pulsed DCs. The mean number of IFN-γ ELISpot ± SEM. is shown (n = 2–3).

    Article Snippet: Briefly, 96-well plates with nitrocellulose membranes (Millipore, Molshelm, France) precoated with a primary anti-IFN-γ antibody were pretreated with RPMI-1640 medium that containrf 10% autologous serum at 4 °C overnight.

    Techniques: Enzyme-linked Immunospot, Concentration Assay, Cytotoxicity Assay, Co-Culture Assay